Recruitment Kinetics of XRCC1 and RNF8 Following MeV Proton and α-Particle Micro-Irradiation

Time-lapse fluorescence imaging coupled to micro-irradiation devices provides information on the kinetics of DNA repair protein accumulation, from a few seconds several minutes after irradiation. Charged-particle microbeams are valuable tools for such studies since they provide way selectively irradiate micrometric areas within cell nucleus, control dose and micro-dosimetric quantities by means advanced detection systems Monte Carlo simulations monitor early response beamline microscopy. We used charged-particle microbeam installed at AIFIRA facility perform experiments measure recruitment two proteins involved in signaling pathways following exposure protons α-particles. developed validated image acquisition processing methods enable systematic study GFP-XRCC1 GFP-RNF8. show that XRCC1 is recruited damage sites irradiation as function total deposited energy quite independently particle LET. RNF8 its depends